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Coding

Part:BBa_K351004:Design

Designed by: Dongchan Yang   Group: iGEM10_KAIST-Korea   (2010-07-22)

Modified Human FGF receptor for MPT51 antigen with fission yeast surface signal peptide


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 685
    Illegal PstI site found at 175
    Illegal PstI site found at 480
    Illegal PstI site found at 1741
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1392
    Illegal SpeI site found at 685
    Illegal PstI site found at 175
    Illegal PstI site found at 480
    Illegal PstI site found at 1741
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2099
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 685
    Illegal PstI site found at 175
    Illegal PstI site found at 480
    Illegal PstI site found at 1741
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 685
    Illegal PstI site found at 175
    Illegal PstI site found at 480
    Illegal PstI site found at 1741
    Illegal NgoMIV site found at 1119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1401
    Illegal BsaI.rc site found at 1889
    Illegal BsaI.rc site found at 2253


Design Notes

This sequence contain signal peptide for fission yeast. You can't use this sequence directly for other organism like budding yeast.


Source

Signal petpdie is from Cell wall integrity and stress response component 1 of Fission yeast. Protein is from Normal human FGFR sequence without signal peptide. MPT51 binding domain is from "single chain antibody for Mycobacterium tuberculosis MPT51 antigen".

References